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Assay validation of the EIA kit; results show the precision (intra-assay coefficient of variation (CV)), specificity (dilution test), accuracy (spike-and-recovery test), and sensitivity of the measurement of reproductive hormones in skin biopsies of males and females of small-spotted catshark ( Scyliorhinus canicula ). Results are shown as mean (±SD).
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Assay validation of the EIA kit; results show the precision (intra-assay coefficient of variation (CV)), specificity (dilution test), accuracy (spike-and-recovery test), and sensitivity of the measurement of reproductive hormones in skin biopsies of males and females of small-spotted catshark ( Scyliorhinus canicula ). Results are shown as mean (±SD).

Journal: Animals : an Open Access Journal from MDPI

Article Title: A New Research Tool for Use in Sharks and Rays: Relevance of Reproductive Hormone Levels in the Skin of Small-Spotted Catshark ( Scyliorhinus canicula )

doi: 10.3390/ani15050762

Figure Lengend Snippet: Assay validation of the EIA kit; results show the precision (intra-assay coefficient of variation (CV)), specificity (dilution test), accuracy (spike-and-recovery test), and sensitivity of the measurement of reproductive hormones in skin biopsies of males and females of small-spotted catshark ( Scyliorhinus canicula ). Results are shown as mean (±SD).

Article Snippet: Commercial enzyme immunoassays of testosterone, estradiol, and progesterone (EIA KIT; Neogen ® Corporation, Ayr, UK) were used to quantify hormone levels in skin extracts of small-spotted catshark.

Techniques: Biomarker Discovery, Intra Assay

Skin progesterone ( A ), 17β-estradiol ( B ), and testosterone ( C ) levels (pg/mg) in females (N = 21) and immature (N = 6) and mature (N = 14) males of small-spotted catshark ( Scyliorhinus canicula ). The boxplot displays the median value and the interquartile range (25th and 75th percentiles). Whiskers present the highest and lowest values in the dataset. Asterisks represent significant differences between studied groups.

Journal: Animals : an Open Access Journal from MDPI

Article Title: A New Research Tool for Use in Sharks and Rays: Relevance of Reproductive Hormone Levels in the Skin of Small-Spotted Catshark ( Scyliorhinus canicula )

doi: 10.3390/ani15050762

Figure Lengend Snippet: Skin progesterone ( A ), 17β-estradiol ( B ), and testosterone ( C ) levels (pg/mg) in females (N = 21) and immature (N = 6) and mature (N = 14) males of small-spotted catshark ( Scyliorhinus canicula ). The boxplot displays the median value and the interquartile range (25th and 75th percentiles). Whiskers present the highest and lowest values in the dataset. Asterisks represent significant differences between studied groups.

Article Snippet: Commercial enzyme immunoassays of testosterone, estradiol, and progesterone (EIA KIT; Neogen ® Corporation, Ayr, UK) were used to quantify hormone levels in skin extracts of small-spotted catshark.

Techniques:

Correlation between skin hormone levels (progesterone ( A ), 17β-estradiol, ( B ) and testosterone ( C )) and Fulton condition of females (N = 21) and immature (N = 6) and mature (N = 14) males of small-spotted catshark ( Scyliorhinus canicula ).

Journal: Animals : an Open Access Journal from MDPI

Article Title: A New Research Tool for Use in Sharks and Rays: Relevance of Reproductive Hormone Levels in the Skin of Small-Spotted Catshark ( Scyliorhinus canicula )

doi: 10.3390/ani15050762

Figure Lengend Snippet: Correlation between skin hormone levels (progesterone ( A ), 17β-estradiol, ( B ) and testosterone ( C )) and Fulton condition of females (N = 21) and immature (N = 6) and mature (N = 14) males of small-spotted catshark ( Scyliorhinus canicula ).

Article Snippet: Commercial enzyme immunoassays of testosterone, estradiol, and progesterone (EIA KIT; Neogen ® Corporation, Ayr, UK) were used to quantify hormone levels in skin extracts of small-spotted catshark.

Techniques: